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    Mar 14, 2022 · The reaction was run on ABI Step One qPCR system (Applied Biosystem, USA) as follows: initial step at 95°C for 15 min, followed by 40 cycles at 95°C for 10 s, 60°C for 30 s. Eighteen seconds rRNA was chosen as internal reference gene for fungi and 16s rRNA for bacteria to normalize the data respectively.Get Price